Katerina Akassoglou, PhD, University of California San Francisco, San Francisco, CA, discusses the value of in vivo two-photon imaging as a technique to study microglial function in health and disease. Two-photon microscopy is a powerful tool for monitoring the live activity of microglia in the brain and spinal cord in disease models. Given the diverse roles of microglia and their highly dynamic responses to external stimuli, the ability to image the dynamics of microglia and their interactions is of great value. Dr Akassoglou explains that her work so far has predominantly focused on morphological changes in disease models and her next steps are to investigate how microglia interact with neuronal cells in different disease states. This interview took place at the American Epilepsy Society (AES) Annual Meeting 2022 in Nashville, TN.
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